Fluorescent In Situ Hybridization Probe Design

Fluorescence in situ hybridization fish is a technique that uses fluorescent probes which bind to special sites of the chromosome with a high degree of sequence complementarity to the probes.

Fluorescent in situ hybridization probe design.

Using spectrally distinct fluorophore labels for each hybridization probe this approach gives you the power to resolve several genetic elements or multiple gene expression patterns through multicolor visual display. Multiplex fluorescence in situ hybridization fish enables you to assay multiple targets and visualize colocalized signals in a single specimen. Where the insert may contain a specific gene or originate from a specific chromosomal. The fluorescent probes are nucleic acid labeled with fluorescent groups and can bind to specific dna rna sequences.

Due to the high allele diversity of genes a single probe is not sufficient to detect all alleles of a gene. The dna probe and the target dna to which the probe will be hybridized. New trends in fluorescence in situ hybridization for identification and functional analyses of microbes. Fluorescence in situ hybridization fish of genes and mrna is most often based on polynucleotide probes.

Wagner m haider s. The dna probe typically comes from cloned sources such as plasmids cosmids pacs yacs or bacs. Fluorescence in situ hybridization fish involves the preparation of two main components.