Flow Cytometry Stem Cell Enumeration

The cd34 antigen is stage specific and identifies cells in the early stages of hematopoietic differentiation.

Flow cytometry stem cell enumeration.

Centers for disease control. Hematopoietic progenitor cells or hpcs are used to reconstitute hematopoiesis following marrow ablative therapies. Issues in enumeration applications of flow cytometry in stem cell research and tissue regeneration 10 1002 9780470631119 115 134 2012. In the present study we review and compare the major counting techniques of stem and progenitor cells.

Bd stem cell enumeration starter kit includes software for first time users. Flow cytometric enumeration of cd34 hematopoietic stem and progenitor cells hscs is widely used for evaluation of graft adequacy of peripheral blood and bone marrow stem cell grafts. Welcome to this pearl of laboratory medicine on the enumeration of cd34 hematopoietic progenitor cells by flow cytometry slide 2. For the analysis of cd34 hscs cell samples are stained with fluorescently labeled antibodies against both the hsc antigen cd34 and the pan leukocyte antigen cd45.

Furthermore cd34 cell enumeration is useful for monitoring the stem cell yield during graft manipulations such as immunomagnetic selections ex vivo expansion or genetic modifications of hscs. I am a development technologist for the human cellular therapy laboratory at mayo clinic. Identification of cd34 hematopoietic stem cells cd34 cell enumeration by flow cytometry is a rare event analysis. A variation on a single platform flow cytometric method based on the ishage gating strategy.

Cytokine and chemokine profiling. Several flow cytometric studies have shown. The advent and use of a cd34 monoclonal antibody enabled the detection of these cells by flow cytometry. Detection of minimal residual disease.

Some of the cytometry tests we offer. Immunophenotyping of blood products. Robert sutherland hematopoietic stem cells. Enumeration of cd34 cells in cord blood.